To prepare 10 ml add 30 µl triton x 100 to 10 ml 1x pbs.
Cdk2 antibody cell signaling.
Red signal from loading control ab8226 observed at 42 kda lanes 5 and 6.
Western blot analysis was performed using cdk12 antibody.
Antibodies are purified by protein a and peptide affinity chromatography.
Cyclin dependent kinase 2 p33cdk2 is an important component of the cell cycle machinery.
Cdk2 cell division protein kinase 2 cyclin dependent kinase 2 cdc2 related protein kinase cell devision kinase 2 p33 protein kinase p33 cdk2 immunogen.
Inhibitory phosphorylation occurs on thr14 and tyr15 1.
The antibody weakly cross reacts with cdc2 phosphorylated at thr161.
Antibody dilution buffer 1x pbs 1 bsa 0 3 triton x 100.
An antibody shouldn t be one of the variables in your experiment.
Ab32147 was shown to specifically react with cdk2 when cdk2 knockout samples were used.
Immunoprecipitation of cdk12 from pc 3 cell extracts using normal rabbit igg 2729 lane 2 or cdk12 antibody lane 3.
Anti rabbit igg h l f ab 2 fragment alexa fluor 488 conjugate 4412.
Like p34cdc2 kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a cdk inhibitor.
Kinase activity associated with cyclin a cdc2 is found only in g2 phase.
It does not cross react with other phosphorylated cyclin dependent kinases.
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Cdk2 antibodies cdk2 antibodies p33cdk2 associates with cyclin a in human cells.
Lane 1 is 10 input.
Phospho cdk2 thr160 antibody detects endogenous levels of cdk2 only when phosphorylated at threonine 160.
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding thr160 of human cdk2.
Like p34cdc2 kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a cdk inhibitor.
Like p34cdc2 kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a cdk inhibitor.
Inhibitory phosphorylation occurs on thr14 and tyr15 1.
Cyclin dependent kinase 2 p33cdk2 is an important component of the cell cycle machinery.
Lanes 1 3 and 5.
Merged red and green signal.
Wild type hap1 cell lysate 20 µg lanes 2 4 and 6.
Mix well then add 0 1 g bsa mix.
Green signal from target ab32147 observed at 34 kda lanes 3 and 4.
Recommended fluorochrome conjugated anti rabbit secondary antibodies.
